Table of Contents Author Guidelines Submit a Manuscript
Journal of Nucleic Acids
Volume 2012, Article ID 672536, 11 pages
http://dx.doi.org/10.1155/2012/672536
Research Article

Functional Annotation of Small Noncoding RNAs Target Genes Provides Evidence for a Deregulated Ubiquitin-Proteasome Pathway in Spinocerebellar Ataxia Type 1

1Department of Medical Genetics, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands
2Animal Science Department, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands
3Department of Comparative Genetics and Refinement, Biomedical Primate Research Center, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands

Received 14 May 2012; Accepted 30 July 2012

Academic Editor: Kenneth K. W. To

Copyright © 2012 Stephan Persengiev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Luciferase assay of HECTD13ÚTR/miR-E1016 and miR-E1108 cotransfections in HeLa cells.

Supplementary Figure 2. miRNA E1108 and miR-E1016 primary genes and processed sequences.

Supplementary Table 1. List of novel small non-coding RNAs (miRNAs) with elevated expression levels in the aging human cortex and cerebellum of healthy individuals and SCA1 patients.

Supplementary Table 2. List of novel small non-coding RNAs (miRNAs) with elevated expression levels in the aging human cortex and cerebellum of healthy individuals.

Supplementary Table 3. Quantitative comparison of the novel upregulated ncRNAs that target ubiquitin-protein processing genes in the cortex and cerebellum of healthy aged individuals and SCA1 patients.

Supplementary Table 4. List of primers used for RT-PCR analysis and HECTD 3’UTR cloning.

Supplementary Table 5. Summary of subject biological parameters and clinical history.

Supplementary Table 6. Gene ontology (GO) annotation of ncRNA target genes in SCA1 cerebellum.

Supplementary Table 7. GO annotation of ncRNA target genes in SCA 1 frontal cortex.

Supplementary Table 8. Gene ontology (GO) annotation of ncRNA target genes in the cerebellum of healthy aged individuals.

Supplementary Table 9. Gene ontology (GO) annotation of ncRNA target genes in the frontal cortex of healthy aged individuals.

  1. Supplementary Materials