Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNA/LNA-Based Apolipoprotein B Antisense Inhibitors
In vitro silencing properties of BNA-based AONs. (a) Various concentrations (15.6 pM–64 nM) of AONs were introduced into Huh-7 cells using Lipofectamine 2000. After 24-h incubation, the cells were collected, and the expression levels of apoB mRNA were determined. Data represent means SD. (b) Reduction of Apo B protein levels in the culture medium following transfection was confirmed by western blotting.
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