Superior Silencing by 2′,4′-BNA^NC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNA/LNA-Based Apolipoprotein B Antisense Inhibitors

Table 1

Oligonucleotides used in this study.

ID	Sequence	𝑇𝑚 (°C)	IC50 (nM)
ApoB-LNA-20	5′-TTCAGcattggtattCAGTG-3′	75 ± 0.8	7.9 ± 1.7
ApoB-LNA-16	5′-CAGcattggtatTCAG-3′	64 ± 0.8	2.9 ± 0.4^a
ApoB-LNA-14	5′-AGCattggtatTCA-3′	61 ± 0.2	1.4 ± 0.5^b
ApoB-LNA-13	5′-GCattggtatTCA-3′	58 ± 0.2	2.8 ± 0.8^a
ApoB-LNA-10	5′-CattggtatT-3′	25 ± 1.4	N.D.
ApoB-NC-20	5′-TTCAGcattggtattCAGTG-3′	79 ± 1.0	11.4 ± 3.0
ApoB-NC-16	5′-CAGcattggtatTCAG-3′	69 ± 0.8	1.3 ± 0.3^b
ApoB-NC-14	5′-AGCattggtatTCA-3′	64 ± 0.3	0.6 ± 0.1
ApoB-NC-13	5′-GCattggtatTCA-3′	61 ± 0.1	0.9 ± 0.1
ApoB-NC-10	5′-CattggtatT-3′	32 ± 0.4	N.D.

2′,4′-BNA/LNA was shown in uppercase and 2′,4′-BNA^NC  was in italic. Natural DNA was shown in lowercase. All the linkages are phosphorothioated. We measured 𝑇_𝑚 and IC₅₀ values of all entries. 𝑇_𝑚 values were determined in three independent experiments (±SD). Nondetectable IC₅₀ values, due to low potency, were marked ND. ^a,bPairs of two IC₅₀ values with superscript letters are NOT statistically significant.