Alternative Splicing of Fibroblast Growth Factor Receptor IgIII Loops in Cancer
Figure 4
IIIb/IIIc splice reporter systems. Schematic of bichromatic fluorescence reporter constructs for IIIc (a) and IIIb/IIIc (b) analyses with adaptations from [90] and [92]. The reporter genes are positioned downstream of the splicing cassette (bold) in two different reading frames, each containing an individual stop codon. Inclusion of exon IIIc (and for b simultaneous skipping of IIIb) of FGFR2 results in a fusion protein in frame with EGFP (green) ending at stop codon 2. Skipping of this exon (and for b simultaneous inclusion of IIIb) results in DsRED (red) expression in another reading frame which contains stop codon 1. Possible adaptations of this splicing reporter system are indicated to exchange (1) species specific orthologous sequences, (2) sequences from IIIb splice variants, (3) sequences from paralogous FGFR 1 and 3, and (4) sequences for other fluorescence reporter proteins.
