Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
Figure 4
Comparison of different cZIPs linked to identical miRNA oligonucleotides based on synthetic mature (a) and precursor (b) miRNA template labeling. Using identical miRNA-specific oligonucleotides with different cZIPs in separate Eppendorf cups, mature synthetic miRNA templates were labeled with Cy3-dUTP and unlabeled dTTP (a), or precursor synthetic miRNA templates were labeled with Cy3-dATP and unlabeled deoxynucleotides (b). Log signal intensities from subsequent hybridization to the ZIP-code array in triplicates are shown. Different cZIPs for identical miRNA are illustrated contiguous in identical colors.
