Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
Table 1
Sequences of mature and precursor synthetic miRNA templates. Synthetic miRNA templates composed of reverse complementary miRNA sequence (bold), 14-mer poly d(T) sequence (standard) and reverse complementary T7 RNA polymerase promoter sequence (italic). Synthetic templates of four precursor synthetic miRNAs were adapted to a maximum length of 80 bases*.
miRBase ID
miRBase accession number
Sequence (5′ to 3′ orientation)
hsa-miR-9-5p
MIMAT0000441
TCA TAC AGC TAG ATA ACC AAA GA TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-9-3
MI0000468
CA CTC ATA CAG CTA GAT AAC CAA AGA GAG AAA CGG GCCTCC TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-16-5p
MIMAT0000069
CGC CAA TAT TTA CGT GCT GCT A TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-16-1
MI0000070
TAA CGC CAA TAT TTA CGT GCT GCT AAG GCA CTG CTG AC TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-92a-3p
MIMAT0000092
ACA GGC CGG GAC AAG TGC AAT A TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-92a-1*
MI0000093
TCA ACA GGC CGG GAC AAG TGC AAT ACC ATA CAG AAA CA TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-100-5p
MIMAT0000098
CAC AAG TTC GGA TCT ACG GGT T TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-100
MI0000102
TA CCA CAA GTT CGG ATC TAC GGG TTT GTG GCA ACA GG TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-19b-3p
MIMAT0000074
TCA GTT TTG CAT GGA TTT GCA CA TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-19b-1*
MI0000074
C AGT CAG TTT TGC ATG GAT TTG CAC AGC AGA ATA TCA C TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-139-5p
MIMAT0000250
CTG GAG ACA CGT GCA CTG TAG A TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-139
MI0000261
AC ACT GGA GAC ACG TGC ACT GTA GAA TAC AC TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-29a-3p
MIMAT0000086
TAA CCG ATT TCA GAT GGT GCT A TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-29a*
MI0000087
ATA ACC GAT TTC AGA TGG TGC TAG AAA ATT ATA TTG AC TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-199b-5p
MIMAT0000263
GAA CAG ATA GTC TAA ACA CTG GG TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-199b*
MI0000282
C CTG AAC AGA TAG TCT AAA CAC TGG GTA GAC GGA GTG GAG TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-miR-99a-5p
MIMAT0000097
CAC AAG ATC GGA TCT ACG GGT T TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
hsa-mir-99a
MI0000101
C ACC ACA AGA TCG GAT CTA CGG GTT TAT GCC AAT GGG TTTTTTTTTTTTTT CCCTATAGTGAGTCGTATTA
