Research Article

Transcriptionally Repressive Chromatin Remodelling and CpG Methylation in the Presence of Expanded CTG-Repeats at the DM1 Locus

Table 1

List of primers used for expression analysis.

DMPK sense
 primer for RT: CGACTGGAGCACGAGGACACTGACTTGCTCAGCAGTGTCAGCAGGTCCCCGCC
 qPCR primers:
Forward: CGACTGGAGCACGAGGACACTGA
Reverse: GGAGAGGGACGTGTTG
DMPK antisense
 primer for RT: CGACTGGAGCACGAGGACACTGAGACCATTTCTTTCTTTCGGCCAGGCTGAGGC
 qPCR primers: Forward: GGAGCACGAGGACACTGA
Reverse: TGCGAACCAACGATAG
SIX5
 primer for RT: Random hexamers
 qPCR primers:Forward: TGGTGGTGCTGGGGGTTGTATC
Reverse: GGGGCAGGGTGTTCCGCTTAC
Dmpk (specific priming)
 primer for RT: CGACTGGAGCACGAGGACACTGACTCAGCAGCGTTAGCA
 qPCR primers:Forward: GGAAGAAAGGGATGTATTA
Reverse: CTCAGCAGCGTTAGCA
Dmpk (random priming)
 primer for RT: Random hexamers
 qPCR primers:Forward: GGAAGAAAGGGATGTATTA
Reverse: CTCAGCAGCGTTAGCA
18S (specific priming)
 primer for RT:CGGGTTGGTTTTGATCTG
 qPCR primers:Forward: CAGTGAAACTGCGAATGG
Reverse: CGGGTTGGTTTTGATCTG
18S (random priming)
 primer for RT:Random hexamers
 qPCR primers:Forward: CAGTGAAACTGCGAATGG
Reverse: CGGGTTGGTTTTGATCTG
Gapdh (specific priming)
 primer for RT:TGTAGACCATGTAGTTGAGGTCA
 qPCR primers:Forward: AGGTCGGTGAACGGATTTG
Reverse: TGTAGACCATGTAGTTGAGGTCA
Gapdh (random priming)
 primer for RT:Random hexamers
 qPCR primers:Forward: AGGTCGGTGAACGGATTTG
Reverse: TGTAGACCATGTAGTTGAGGTCA