Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis

Figure 5

Data Analysis Pipeline. SOAPsnp was used to find SNPs in the data. The criteria for SNP validation in SOAPsnp is rather low. Variant validation is highly critical in metagenomic samples, more so than with homogeneous samples. False variants created by sequencer error can quickly change the results of forensics analysis in metagenomic samples where lower coverage depth and partial base consensus conditions are expected, whereas base consensus can be demanded in homogeneous sample data sets.