In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil

Figure 2

Structures of molecules used in the SELEX scheme and cross binding assays. (a) Structure of the herbicide and target of selection bromacil. (b) Structure of the bromacil metabolite 6-amino-3-ethyl methyl uracil, the 1st negative target. (c) Structure of the bromacil metabolite 1-methyl uracil, the 2nd negative target. (d) Structure of the herbicide atrazine, used in cross-reactivity binding assays. (e) Structure of the pesticide malathion, used in cross-reactivity binding assays. (f) Ribbon structure of bovine serum albumin (BSA) used in cross binding assays [25].

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