Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation

Figure 2

Extension from primer ends by Pol . Each primer contained a different nucleotide at the 3′ end (indicated by N, where N was C, G, A, or T in lanes 1–3, 4–6, 7–9, and 8–12, resp.) opposite G (a) or Oz (b). The amount of Pol was 25 mU in lanes 1, 4, 7, and 10 or 2.5 mU in lanes 2, 5, 8, and 11.

(a)

(b)