Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation

Figure 3

Extension from primer ends by KF exo⁻. Each primer contained a different nucleotide at the 3′ end (indicated by N, where N was C, G, A, or T in lanes 1–3, 4–6, 7–9, and 8–12, resp.) opposite G (a) or Oz (b). The amount of KF exo⁻ was 250 μU in lanes 1, 4, 7, and 10 or 25 μU in lanes 2, 5, 8, and 11.

(a)

(b)