(a)
(b)
Figure 4: Extension from primer ends by Pol . Each primer contained a different nucleotide at the 3′ end (indicated by N, where N was C, G, A, or T in lanes 1–3, 4–6, 7–9, and 8–12, resp.) opposite G (a) or Oz (b). The amount of Pol was 11.5 ng in lanes 1, 4, 7, and 10 or 1.15 ng in lanes 2, 5, 8, and 11.