Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation

Figure 6

Nucleotide incorporation opposite the bases adjacent to G or Oz by Pol (a), KF exo⁻ (b), or Pol (c). Left panels of (a), (b), and (c) show the control, which was extension of primers containing C opposite an undamaged G in the template. Right panels of (a), (b), and (c) show the extension of primers containing G opposite Oz in the template. The amount of Pol or Pol was 25 mU or 11.5 ng; the amount of KF exo⁻ was 25 μU for the left panel or 250 μU for the right panel.

(a)

(b)

(c)