Research Article

Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins

Table 1

Concentrations of RNA aptamer pool and Stx1 or Stx2 for SELEX selection.

Selection roundMembrane-SELEXPlate-SELEX
Input RNA (pmoles)Input Stx1/Stx2 (pmoles)Input RNA (pmoles)Input Stx1/Stx2 (pmoles)

1160040040028
280040020028
320020020028
420020020028
52005020028
62005020028
72002520028