Research Article
Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins
Table 1
Concentrations of RNA aptamer pool and Stx1 or Stx2 for SELEX selection.
| Selection round | Membrane-SELEX | Plate-SELEX | Input RNA (pmoles) | Input Stx1/Stx2 (pmoles) | Input RNA (pmoles) | Input Stx1/Stx2 (pmoles) |
| 1 | 1600 | 400 | 400 | 28 | 2 | 800 | 400 | 200 | 28 | 3 | 200 | 200 | 200 | 28 | 4 | 200 | 200 | 200 | 28 | 5 | 200 | 50 | 200 | 28 | 6 | 200 | 50 | 200 | 28 | 7 | 200 | 25 | 200 | 28 |
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