Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins

Table 2

Sequences of RNA aptamers selected following membrane- and plate-SELEX.

Group	n ^1	Sequence^2
mA	43	ATTAGCTATCTTCCACGATTCGATCAGGCAGTACGTCGT^3,4
mB	35	ACAGTTATCCGACTGCTATTCGATCAGGCAGTACGTAGC^3,5
mC	6	CAGGCTGTTCTGACGCATAAGGAATGCGCTGTTGCAGAG
mD	4	TTGGTCCTGCTTTGGATAGTCGCGAAAGGGGTGCCACTG
m-Singles	8	Orphan sequences^6
pA	29	ACAGTTATCCGACTGCTATTCGATCAGGCAGTACGTAGC^3,5
pB	18	ACCGAGCGGTTTTACGTCTCAAGTAGTATCCCGTTTTGC
pC	7	ATTAGCTATCTTCCACGATTCGATCAGGCAGTACGTCGT^3,4
pD	4	TTGCCATCCTGTACTATGCTCTATCGGGCGGTTTAGTGATCCTTCGTCCAACTATC
p-Singles	42	Orphan sequences^5

1Number of isolates harboring the represented sequence in each group. ²Complete random sequences of the aptamer core regions (5′-3′) are shown. ³Underlined sequence of 19 bases is identical among groups mA, mB, pA and pC. ⁴Complete sequences of mA and pC groups are identical. ⁵Complete sequences of mB and pA groups are identical. ⁶Orphan sequences are the sequences that are seen only in one isolate.