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Journal of Nucleic Acids
Volume 2014, Article ID 570176, 12 pages
Research Article

Discovery of Novel Leaf Rust Responsive microRNAs in Wheat and Prediction of Their Target Genes

1Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835215, India
2Department of Biotechnology, Eternal University, Baru Sahib, Himachal Pradesh 173101, India
3National Phytotron Facility, IARI, New Delhi 110012, India

Received 21 May 2014; Accepted 19 July 2014; Published 12 August 2014

Academic Editor: Ben Berkhout

Copyright © 2014 Dhananjay Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Triticum aestivum L. pre-miRNA secondary structures predicted using Mfold showing the mature miRNAs in stem portion (green). Gene Ontology (GO) categories and distribution of miRNA target genes in wheat. The results are classified in three main categories: cellular component, molecular function and biological process. The y-axis on the left indicates the percent of genes in a category, while the y-axis on the right indicates the number of genes in a specific category. Gene Ontology (GO) term enrichment analysis of the miRNAs target genes. Analysis of the targetome of miRNAs within Biological process (A) and Molecular function (B) category. The predicted regulatory relationships between miRNA targets were performed by using the online tool Blast2GO with default parameters. Wheat miRNAs identified by homolog search and secondary structure. Potential target genes for the identified miRNAs.

  1. Supplementary Material