In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Figure 7

Modified ELISA assays of toxin B. Data from one modified sandwich ELISA assay with absorbance measured at OD 410 nm. Absorbance levels presented are subtracted from background levels of blank wells without immobilized DNA (negative control). Error bars represent 2x standard deviations of 2 sample replicates. (a) Statistical significance levels with respect to DNA in buffer background (without toxin B) of are designated by **. (b) Statistical significance levels with respect to fecal background (without toxin B) of are designated by *. Buffer: 1x selection buffer; feces: 1 g/20 mL 1x selection buffer.

(a)

(b)