The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding

Figure 3

(a) SHAPE-derived minimum free energy (MFE) 2D structures of the RPS12 pre-mRNA: top, at dilute solvent conditions in the presence of 2 mM Mg²⁺; bottom, at crowded solvent conditions in the presence/absence of 2 mM Mg²⁺. (b) Circle-plot comparison of SHAPE-derived 2D structures of the RPS12 pre-mRNA. Base pairs (bp) are shown as colored lines. Green: bp present in both dilute and crowded solvent conditions. Black: bp unique to dilute solvent conditions in the presence of Mg²⁺. Red: bp unique to the specific solvent conditions.

(a)

(b)