Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage

Figure 1

(a) Top: schematic illustration of experimental protocol for studying the effect of chemicals on gap-filling during repair of UV-induced DNA lesions. Bottom: colcemid delays the gap-filling. Initial level of the gaps at the time zero after H/A removal was taken as 100%. (b) Similar to (a), AGS cells in log phase were UV irradiated (3 J/m²) and H/A treated for 2 h. After H/A was removed, cells were treated with or without colcemid or 18 μM -carotene. Cells were harvested for comet assay at the indicated time. (c) Similar to (b), cells were harvested at 6 h after H/A was removed for comet assay.

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