Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures

Table 4

Comparison of and of transition of iM containing 5-hmC ± KCl (100 mM) and ±40% acetonitrile (ACN) at pH 6.1. The unmodified control iM VEGF sequence () is also shown for comparison. Error bars are omitted for clarity and are <7% or 50% of the values shown for and , respectively.

	Δ (°C)	(kcal/mol)	(°C)	(kcal/mol)	(°C)	(kcal/mol)	(°C)	(kcal/mol)	(°C)	(kcal/mol)
Control	52	16	52	17	53	15	47	12	51	16
100 mM KCl Alone	48	18	49	8	48	7	44	13	49	7
40% ACN Alone	50	20	48	17	50	18	46	12	49	16
100 mM KCl + 40% ACN	40	10	40	9	41	10	40	5	42	12