Figure 2: Kinetic studies indicated that the decrease in fluorescence intensity of YOYO intercalated in different DNAs after the addition of 1.2 μM α-PrP. Traces (a), (b), and (c) are gcDNA, mDNA, and atDNA, respectively. DNA concentrations 1 μM in phosphate, dye 20 nM (dye to phosphate ratio = 1 : 50). fluorescence intensity of the dye before the addition of the prion protein and the fluorescence values after the addition of the protein. Temperature 20°C. Excitation 485 nm; emission 505 nm.