Figure 9: Dependence of fluorescence quenching of gcDNA bound YOYO with varying dye to DNA molar ratio in the presence of increasing α-PrP concentrations. (a) Titration with dye : DNA-ph = 1 : 50 and (b) with dye : DNA-ph = 1 : 2000. Therefore dye : DNA-ph ratio = 1 : 2000 used for the acrylamide accessibility experiments.