Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MS/MS and Comet Assay

Table 1

Statistical analysis of multiple comet assays (% DNA in tail).

Etoposide	Control	1.5 μg/mL	3.0 μg/mL	6.0 μg/mL
Mean (Av. of 3 means)	18.46	18.86	32.92	45.34
Stand Dev.,	0.894	1.190	9.807	8.301
Stnd. error of mean	0.6324	0.8416	6.934	5.870
value (vs. control)		0.3632	0.0533	0.0052
Bleomycin	Control	0.5 μg/mL	1.0 μg/mL	2.0 μg/mL
Mean (Av. of 3 means)	16.75	20.00	24.97	37.22
Stnd. Dev.,	1.331	4.521	2.146	5.784
Stand. error of mean	0.9415	3.197	1.518	4.090
value (vs. control)		0.1925	0.0050	0.0041
EMS	Control	400 μg/mL	800 μg/mL	1600 μg/mL
Mean (Av. of 3 means)	16.88	49.25	69.47	82.02
Stnd. Dev.,	0.278	2.605	2.821	10.335
Stand. error of mean	0.1965	1.845	1.995	7.308
value (vs. control)		<0.0001	<0.0001	0.0004

Statistical calculations are based on three sets of comet assays using aliquots of the same sets of treated samples.