Perturbing the Normal Level of SIDT1 Suppresses the Naked ASO Effect

Figure 6

The SIDT2 level hardly affects the total cellular uptake of naked A-15. MEFs were transfected with the empty plasmid (control) or pCI-neo-SIDT2 for 48 hours. Then, the cells were rinsed with PBS and cultured with or without 500 nM of naked A-15. After the 24-hour culture, the cells were washed with PBS and analyzed with a flow cytometer. In the left and middle panels, yellow and red histograms are from MEFs cultured in the absence and presence, respectively, of A-15. In the right panel, the red histograms in the left and middle panels are overlaid and shown in yellow and red, respectively.