Research Article
Development of a Reference Method and Materials for Quantitative Measurement of UV-Induced DNA Damage in Mammalian Cells: Comparison of Comet Assay and Cell Viability
Figure 3
Loss of cell viability as the level of concurrent UVA and BaP exposure increases. (a) Effect of BaP concentration (10−7 to 10−4) mol/L with 30 min UVA exposure (filled symbols) and without (open symbols). Data from 2 separate replicate experiments is included. (b) Effect of increasing UVA exposure (, , and ) and increasing BaP concentration: media only (▲); 10−7 M (●); 10–6 M (o); 10−5 M (Δ); 10−4 M (x). The data point at 10−4 M BaP, 10 min. UVA exposure was obtained from a different but equivalent set of experiments. UVA exposure times were at an intensity of 3.5 mW/cm2. M: mol/L. Results were calculated and plotted as the mean and standard deviation (see Materials and Methods).
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