Genetic Polymorphisms and Forensic Parameters of Thirteen X-Chromosome Markers in the Iraqi Kurdish Population

Table 1

Table showing locus name and characteristics of the 13 X-STR loci and amelogenin included in the PCR system.

Locus	Cytogenetic localization	Repeat motif	Allele range	Primer sequence (-)	Dye	Fragment size (bp)	TM (°C)
AMEL	X: p 22.1-22.3; Y: p 11.2	—	X, Y	F-CCCTGGGCTCTGTAAAGAATA; R-ATCAGAGCTTAAACTGGGAAGCTG	FAM	106-112	59; 60.9
DXS9902	p 22.2	ATCT	7-13	F-GTACTGTTCTTGTAACTTTTCTGTCG; R-CAG GAG TAT GGG ATCACCAGTGA	FAM	118-148	63.1; 66.3
DXS10164	Centromere	ATTCT	7-15	F-GGCAGTATTAATCAAAACAGAATGG; R-TGAACGGAATGTACTTTTCCAA	FAM	180-224	55.7; 56.6
DXS7130	q 24	TATC	10-18	F-TCCTTCAATTGCCCCATCGATAG; R-GTGCCCAATGAATGCCTCCATA	FAM	265-302	62; 62.3
DXS7423	q 28	TCCA	12-18	F-GCCACTGGGGACATGTGAATGG; R-CACCCAGATTTCCTCCCCA	FAM	302-330	66.9; 64.7
DXS8378	p 22.31	CTAT	6-14	R-GCGACAAGAGCGAAACTCCA; F-GCTCCTGGCAGGTCACTATC	ATTO532	80-118	62.9; 62.3
GATA172D05	q 23	TAGA	4-13	F-TACTGTTTAACCAGTACAAAGTT; R-TCTATAGATATAGGTATTGATATAGC	ATTO532	124-164	55.6; 51.1
DXS9898	q 21.31	TATC	8-15	F-CGAGCACACCTACAAAAGCT; R-TCGATTAGGTTCAGTTCCCA	ATTO532	183-219	59; 58.5
DXS7424	q 22.1	TAA	8-19	F-GAGTCCAGGAATTCAAGGCCT; R-TACAGCTAAGAAGAATCCCGCA	ATTO550	100-138	62.7; 59.7
GATA31E08	q 27.1	AGAT	7-13	F-TGTTTATCATACTAGATATAGATA; R-AGATACATGTATGTATGCTCACT	ATTO550	150-182	53.4; 57.6
DXS6795	p 22.11	AAT	9-17	F-CTGGTCCAACTGATGCACAC; R-GAAATGCATCCATCCCCTAA	ATTO550	214-249	61.2; 57.4
DXS981 (STRX1)	q 13.1	TATC	12-17	F-CTTCTCCAGCACCCAAGGAAGTCA; R-CTCCTTGTGGCCTTCCTTAAATGG	ATTO550	307-334	66.8; 63.5
DXS7132	Centromere	TCTA	11-17	F-ATAAATCCCCTCTCATCTATCTGAC; R-ACTCCTGGTGCCAAACTCTA	ATTO565	128-158	57.7; 60.7
GATA144D04	p 11.23	CTAT	9-17	F-AAGATCTGCCAAGCCAGAAG; R-CTTGCAGTGAGGGACAGAGA	ATTO565	225-262	59.6; 62.1