Glial Cell Line-Derived Neurotrophic Factor Family Members Reduce Microglial Activation via Inhibiting p38MAPKs-Mediated Inflammatory Responses

Figure 5

GFL reduced the secretion of IL-6 and TNF-𝛼. Supernatants of LPS activated microglial cells pretreated with GFL were analyzed by ELISA for the secretion of IL-6 (a) and TNF-𝛼 (b) after 24 hrs. The data were assessed from three independent experiments in triplicate. Asterisks (^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0,001) indicate a significant difference compared with cells stimulated with LPS (ANOVA, followed by the Bonferroni test, 𝑛≥3, LPS=100%).

(a)

(b)