Disturbed Matrix Metalloproteinase Pathway in Both Age-Related Macular Degeneration and Alzheimer’s Disease
Table 2
Brain MMP levels and their relative distribution between bound and mobile compartments.
(a) Gelatinase bands in the zymograms of Figure 2 were quantified by densitometry and activity expressed as gel band area per µg protein (4 controls and 4 AD donors). Level of HMW1 in the homogenate of AD samples showed a significant reduction compared to controls. AD, Alzheimer’s disease; NDet, not detected; .
MMP species
MMP activity (gel band area/μg protein) mean ± SD
Homogenate
Supernatant
Control
AD
Control
AD
HMW2
HMW1
Pro-MMP9
Pro-MMP2
NDet
NDet
(b) For each MMP species in Figure 2, the gel band area per µg of protein was determined for both homogenates and supernatants and the percentage of species bound was calculated using (1). HMW2 was observed to be tightly bound to the membranous or insoluble extracellular component in brain tissue. The amount of HMW1 and pro-MMP9 bound was significantly reduced in AD. Control donors: numbers 1–4; AD donors: numbers 5–7, 9. .
