Table of Contents
Journal of Nanoparticles
Volume 2015, Article ID 829718, 9 pages
http://dx.doi.org/10.1155/2015/829718
Research Article

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

1Materials Chemistry Laboratory, Department of Materials Science, Gulbarga University, Gulbarga, Karnataka 585106, India
2Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru, Karnataka 5600 89, India
3Department of Chemistry, Gulbarga University, Gulbarga, Karnataka 585106, India

Received 28 July 2014; Accepted 5 October 2014

Academic Editor: Amir Kajbafvala

Copyright © 2015 Kantrao Saware et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1- to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.