Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Figure 6

Tryptophan fluorescence quenching spectra of α-amylase with nanoparticles. Line (a) corresponds to amylase alone; Line (b-g) with nanoparticles. (a) Amylase + FB AgNPs; (b) amylase + FR AgNPs; (c) amylase + gold nanoparticles. Decreasing of Trp fluorescence spectra with increasing (5, 10, 15, 20, and 30 μL) concentration of silver and gold nanoparticles. Amylase titrated with increasing concentration of nanoparticles excitation at 280 nm followed emission at 335 nm (slit width for both 5 nm).

(a)

(b)

(c)