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Journal of Nanoscience
Volume 2013 (2013), Article ID 126564, 6 pages
Research Article

Phytochemical Synthesis and Preliminary Characterization of Silver Nanoparticles Using Hesperidin

Department of Pharmacology, Sri Ramachandra Medical College & Research Institute, Chennai, India

Received 28 August 2013; Accepted 12 November 2013

Academic Editor: Kalimuthu Kalishwaralal

Copyright © 2013 Anish Stephen and Sankar Seethalakshmi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper is the first of its kind for development of rapid and ecofriendly method for synthesis of silver nanoparticles from aqueous solution of silver nitrate using the flavonoid “hesperidin” and optimization of the methodology. There is formation of stable spherical silver nanoparticles in the size range of 20–40 nm. Optimization of methodology in terms of concentration of reactants and pH of the reaction mixture reduced the reaction time for silver nanoparticle formation to 2 mins. Silver nanoparticles (AgNPs) were characterized by UV-Vis spectroscopy and transmission electron microscopy (TEM). UV-vis spectroscopy derived spectrum demonstrated a peak of 430 nm which corresponds to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy revealed spherical shaped silver nanoparticles in the size range of 20–40 nm.