Table of Contents
Journal of Nanoscience
Volume 2016, Article ID 4023852, 9 pages
http://dx.doi.org/10.1155/2016/4023852
Research Article

In Vitro Cytotoxicity of Nanoparticles: A Comparison between Particle Size and Cell Type

1Pharmacology and Toxicology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
2School of Studies in Pharmaceutical Sciences, Jiwaji University, Gwalior 474011, India
3Saveetha University, Chennai 600077, India

Received 14 March 2016; Revised 15 June 2016; Accepted 30 June 2016

Academic Editor: Nikolai Khlebtsov

Copyright © 2016 Devashri Sahu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. S. Edelstein and R. C. Cammaratra, Nanomaterials: Synthesis, Properties and Applications, CRC Press, New York, NY, USA, 2nd edition, 1998.
  2. N. Staggers, T. McCasky, N. Brazelton, and R. Kennedy, “Nanotechnology: the coming revolution and its implications for consumers, clinicians, and informatics,” Nursing Outlook, vol. 56, no. 5, pp. 268–274, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. S. J. Klaine, P. J. J. Alvarez, G. E. Batley et al., “Nanomaterials in the environment: behavior, fate, bioavailability, and effects,” Environmental Toxicology and Chemistry, vol. 27, no. 9, pp. 1825–1851, 2008. View at Publisher · View at Google Scholar · View at Scopus
  4. P. Schulte, C. Geraci, R. Zumwalde, M. Hoover, and E. Kuempel, “Occupational risk management of engineered nanoparticles,” Journal of Occupational and Environmental Hygiene, vol. 5, no. 4, pp. 239–249, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. Z. Fan and J. G. Lu, “Zinc oxide nanostructures: synthesis and properties,” Journal of Nanoscience and Nanotechnology, vol. 5, no. 10, pp. 1561–1573, 2005. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Balduzzi, M. Diociaiuti, B. De Berardis, S. Paradisi, and L. Paoletti, “In vitro effects on macrophages induced by noncytotoxic doses of silica particles possibly relevant to ambient exposure,” Environmental Research, vol. 96, no. 1, pp. 62–71, 2004. View at Publisher · View at Google Scholar · View at Scopus
  7. P. H. M. Hoet, I. Brüske-Hohlfeld, and O. V. Salata, “Nanoparticles—known and unknown health risks,” Journal of Nanobiotechnology, vol. 2, article 12, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. I. Roy, T. Y. Ohulchanskyy, D. J. Bharali et al., “Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 2, pp. 279–284, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. Z. S. Csogor, M. Nacken, M. Sameti, C.-M. Lehr, and H. Schmidt, “Modified silica particles for gene delivery,” Materials Science and Engineering: C, vol. 23, no. 1-2, pp. 93–97, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. N. Lewinski, V. Colvin, and R. Drezek, “Cytotoxicity of nanoparticles,” Small, vol. 4, no. 1, pp. 26–49, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, “Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells,” Nano Letters, vol. 6, no. 4, pp. 662–668, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. C. J. Murphy, A. M. Gole, J. W. Stone et al., “Gold nanoparticles in biology: beyond toxicity to cellular imaging,” Accounts of Chemical Research, vol. 41, no. 12, pp. 1721–1730, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Nan, X. Bai, S. J. Son, S. B. Lee, and H. Ghandehari, “Cellular uptake and cytotoxicity of silica nanotubes,” Nano Letters, vol. 8, no. 8, pp. 2150–2154, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. B. D. Chithrani and W. C. W. Chan, “Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes,” Nano Letters, vol. 7, no. 6, pp. 1542–1550, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Wang, W. Lu, O. Tovmachenko, U. S. Rai, H. Yu, and P. C. Ray, “Challenge in understanding size and shape dependent toxicity of gold nanomaterials in human skin keratinocytes,” Chemical Physics Letters, vol. 463, no. 1–3, pp. 145–149, 2008. View at Publisher · View at Google Scholar
  16. Y. Pan, S. Neuss, A. Leifert et al., “Size-dependent cytotoxicity of gold nanoparticles,” Small, vol. 3, no. 11, pp. 1941–1949, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. X. Q. Zhang, L. H. Yin, M. Tang, and Y. P. Pu, “ZnO, TiO2, SiO2, and Al 2O3 nanoparticles-induced toxic effects on human fetal lung fibroblasts,” Biomedical and Environmental Sciences, vol. 24, no. 6, pp. 661–669, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. H. Yang, C. Liu, D. Yang, H. Zhang, and Z. Xi, “Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition,” Journal of Applied Toxicology, vol. 29, no. 1, pp. 69–78, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. B. Díaz, C. Sánchez-Espinel, M. Arruebo et al., “Assessing methods for blood cell cytotoxic responses to inorganic nanoparticles and nanoparticle aggregates,” Small, vol. 4, no. 11, pp. 2025–2034, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Lanone, F. Rogerieux, J. Geys et al., “Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines,” Particle and Fibre Toxicology, vol. 6, article 14, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Kroll, C. Dierker, C. Rommel et al., “Cytotoxicity screening of 23 engineered nanomaterials using a test matrix of ten cell lines and three different assays,” Particle and Fibre Toxicology, vol. 8, no. 1, article 9, 2011. View at Publisher · View at Google Scholar
  22. C. M. Sayes, K. L. Reed, and D. B. Warheit, “Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles,” Toxicological Sciences, vol. 97, no. 1, pp. 163–180, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. D. M. Brown, M. R. Wilson, W. MacNee, V. Stone, and K. Donaldson, “Size-dependent proinflammatory effects of ultrafine polystyrene particles: a role for surface area and oxidative stress in the enhanced activity of ultrafines,” Toxicology and Applied Pharmacology, vol. 175, no. 3, pp. 191–199, 2001. View at Publisher · View at Google Scholar · View at Scopus
  24. B. Wang, W.-Y. Feng, T.-C. Wang et al., “Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice,” Toxicology Letters, vol. 161, no. 2, pp. 115–123, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. H. L. Karlsson, J. Gustafsson, P. Cronholm, and L. Möller, “Size-dependent toxicity of metal oxide particles-A comparison between nano- and micrometer size,” Toxicology Letters, vol. 188, no. 2, pp. 112–118, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. K. Midander, P. Cronholm, H. L. Karlsson et al., “Surface characteristics, copper release, and toxicity of nano- and micrometer-sized copper and copper(ll) oxide particles: a cross-disciplinary study,” Small, vol. 5, no. 3, pp. 389–399, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. D. B. Warheit, C. M. Sayes, and K. L. Reed, “Nanoscale and fine zinc oxide particles: can in vitro assays accurately forecast lung hazards following inhalation exposures?” Environmental Science and Technology, vol. 43, no. 20, pp. 7939–7945, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. Y. Li, L. Sun, M. Jin et al., “Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells,” Toxicology in Vitro, vol. 25, no. 7, pp. 1343–1352, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. W. Lin, Y.-W. Huang, X.-D. Zhou, and Y. Ma, “In vitro toxicity of silica nanoparticles in human lung cancer cells,” Toxicology and Applied Pharmacology, vol. 217, no. 3, pp. 252–259, 2006. View at Publisher · View at Google Scholar · View at Scopus
  30. W. Lin, Y. Xu, C.-C. Huang et al., “Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells,” Journal of Nanoparticle Research, vol. 11, no. 1, pp. 25–39, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. Y. Ye, J. Liu, M. Chen, L. Sun, and M. Lan, “In vitro toxicity of silica nanoparticles in myocardial cells,” Environmental Toxicology and Pharmacology, vol. 29, no. 2, pp. 131–137, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Prach, V. Stone, and L. Proudfoot, “Zinc oxide nanoparticles and monocytes: impact of size, charge and solubility on activation status,” Toxicology and Applied Pharmacology, vol. 266, no. 1, pp. 19–26, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. M. Ahamed, M. J. Akhtar, M. Raja et al., “Zinc oxide nanorod induced apoptosis via p53, bax/bcl-2 and survivin pathways in human lung cancer cells: role of oxidative stress,” International Journal of Nanomedicine, vol. 7, pp. 845–857, 2012. View at Google Scholar
  34. F. Tian, T. Zhu, and Y. Shang, “Intracellular influx of calcium induced by quartz particles in alveolar macrophages,” Toxicology and Applied Pharmacology, vol. 242, no. 2, pp. 173–181, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. K. M. Waters, L. M. Masiello, R. C. Zangar et al., “Macrophage responses to silica nanoparticles are highly conserved across particle sizes,” Toxicological Sciences, vol. 107, no. 2, pp. 553–569, 2009. View at Publisher · View at Google Scholar · View at Scopus
  36. J. Zhang, W. Song, J. Guo et al., “Toxic effect of different ZnO particles on mouse alveolar macrophages,” Journal of Hazardous Materials, vol. 219-220, pp. 148–155, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. F. Denizot and R. Lang, “Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability,” Journal of Immunological Methods, vol. 89, no. 2, pp. 271–277, 1986. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. T. He, J. Wan, and T. Tokunaga, “Kinetic stability of hematite nanoparticles: the effect of particle sizes,” Journal of Nanoparticle Research, vol. 10, no. 2, pp. 321–332, 2008. View at Publisher · View at Google Scholar · View at Scopus
  39. A. R. Petosa, D. P. Jaisi, I. R. Quevedo, M. Elimelech, and N. Tufenkji, “Aggregation and deposition of engineered nanomaterials in aquatic environments: role of physicochemical interactions,” Environmental Science and Technology, vol. 44, no. 17, pp. 6532–6549, 2010. View at Publisher · View at Google Scholar · View at Scopus
  40. K. Soto, K. M. Garza, and L. E. Murr, “Cytotoxic effects of aggregated nanomaterials,” Acta Biomaterialia, vol. 3, no. 3, pp. 351–358, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. I. Papageorgiou, C. Brown, R. Schins et al., “The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts in vitro,” Biomaterials, vol. 28, no. 19, pp. 2946–2958, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Park, Y. K. Lee, M. Jung et al., “Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells,” Inhalation Toxicology, vol. 19, no. 1, pp. 59–65, 2007. View at Publisher · View at Google Scholar · View at Scopus
  43. X. Deng, Q. Luan, W. Chen et al., “Nanosized zinc oxide particles induce neural stem cell apoptosis,” Nanotechnology, vol. 20, no. 11, article 115101, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. J. Weyermann, D. Lochmann, and A. Zimmer, “A practical note on the use of cytotoxicity assays,” International Journal of Pharmaceutics, vol. 288, no. 2, pp. 369–376, 2005. View at Publisher · View at Google Scholar · View at Scopus