Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography

Figure 2

A typical chromatogram of a mixture of ALT (100 μg mL⁻¹), CDB (300 μg mL⁻¹), and CDZ (100 μg mL⁻¹) at retention times 3.667, 4.427, and 5.233 min, respectively. Chromatographic conditions: RP-HPLC on C₈ column; mobile phase: acetonitrile-methanol-ammonium acetate 0.1 M (30 : 40 : 30, v/v/v); flow rate 1.0 mL min⁻¹ and detection at 218 nm.