Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography

Table 4

Results of robustness study.

Factor	Level	Mean % assay (𝑛=3)		% RSD of results
		Clidinium bromide	Chlordiazepoxide	Clidinium bromide	Chlordiazepoxide
pH of mobile phase	5.1	100.3	100.5	1.32	1.25
	4.9	100.1	100.7	0.86	0.69
Flow rate (mL/min)	0.9	99.9	100.1	0.97	1.04
	1.1	100.2	100.8	0.58	0.53
% of acetonitrile	25	99.7	100.04	1.24	0.95
	35	100.6	101.05	0.47	0.73
% of methanol	35	100.4	100.6	0.86	0.49
	45	100.6	100.9	0.79	0.61