Intranasal, siRNA Delivery to the Brain by TAT/MGF Tagged PEGylated Chitosan Nanoparticles
Figure 3
(a) Histopathological images of brain tissue (cerebral cortex and cerebellum) 4 hrs after receiving the nanoparticle formulation carrying doses of biotin-siRNA: (A) 0.25 mg/kg, (B) 0.5 mg/kg, (C) 1 mg/kg, (D) 2 mg/kg, and (E) control. (b) Quantitative analysis of the stained area in tissues using Image J. This study proved that the novel nanoparticle formulation successfully delivered the biotin-siRNA with high efficiency and selective targeting. The optimal dose of siRNA delivered via nanoparticles was determined to be 0.5 mg/kg. (c) Histopathological images of the cerebral cortex and cerebellum with nanoparticles carrying 0.5 mg/kg of biotin-siRNA at different time points, (A) 4 hrs, (B) 16 hrs, (C) 24 hrs, and (D) 48 hrs. (d) Quantitative analysis of the stained area in tissues using Image J. This study confirmed successful delivery of biotin-siRNA to the brain within 4 hrs of intranasal administration, with its clearance after 16 h. The graph shows a representative result of independent readings from two animals in each group () mean ± s.d. *** was considered highly significant based on Tukey’s post hoc analysis, when compared with other groups.
