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Journal of Pharmaceutics
Volume 2014, Article ID 864901, 8 pages
http://dx.doi.org/10.1155/2014/864901
Research Article

Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India

Received 19 November 2013; Revised 20 December 2013; Accepted 26 December 2013; Published 6 March 2014

Academic Editor: Massoud Amanlou

Copyright © 2014 Kranti P. Musmade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, precise, accurate, rapid, and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated for quantification of naringin (NAR) in novel pharmaceutical formulation. NAR is a polyphenolic flavonoid present in most of the citrus plants having variety of pharmacological activities. Method optimization was carried out by considering the various parameters such as effect of pH and column. The analyte was separated by employing a C18 (?mm, 5?µm) column at ambient temperature in isocratic conditions using phosphate buffer pH 3.5: acetonitrile (75?:?25%?v/v) as mobile phase pumped at a flow rate of 1.0?mL/min. UV detection was carried out at 282 nm. The developed method was validated according to ICH guidelines Q2(R1). The method was found to be precise and accurate on statistical evaluation with a linearity range of 0.1 to 20.0?µg/mL for NAR. The intra- and interday precision studies showed good reproducibility with coefficients of variation (CV) less than 1.0%. The mean recovery of NAR was found to be 99.33 ± 0.16%. The proposed method was found to be highly accurate, sensitive, and robust. The proposed liquid chromatographic method was successfully employed for the routine analysis of said compound in developed novel nanopharmaceuticals. The presence of excipients did not show any interference on the determination of NAR, indicating method specificity.