Novel HPLC Analysis of Hydrocortisone in Conventional and Controlled-Release Pharmaceutical Preparations

Table 1

Optimized chromatographic conditions for the developed HPLC method.

Parameters	Optimized conditions
Mobile phase	Methanol/water/acetic acid (60 : 30 : 10, v/v/v)
HPLC column	JT Baker ODS C18, 5 m, 4.6 × 150 mm
Flow rate	1 ml/min
Wavelength detection	254 nm
Diluent	Methanol
Injection volume	20