Table of Contents
Journal of Polymers
Volume 2013, Article ID 523941, 6 pages
http://dx.doi.org/10.1155/2013/523941
Research Article

Phototrophic Growth and Accumulation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Purple Nonsulfur Bacterium Rhodopseudomonas palustris SP5212

1Department of Microbiology, Lady Brabourne College, Kolkata, West Bengal 700 017, India
2Department of Chemistry, University of Calcutta, Kolkata, West Bengal 700 009, India
3Microbiology Laboratory, Department of Botany, University of Calcutta, Kolkata, West Bengal 700 019, India

Received 27 March 2013; Accepted 13 September 2013

Academic Editor: Changwoon Nah

Copyright © 2013 M. Mukhopadhyay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The ability of the phototrophic bacterium Rhodopseudomonas palustris SP5212 to produce polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] in particular was, assessed in acetate medium supplemented with hydroxybutyrate and valerate as cosubstrates. The isolate accumulated the polymer accounting for some 49.06% and 30% of cell dry weight when grown in hydroxybutyrate and valerate, respectively. PHA accumulation as well as 3HV monomer incorporation (30 mol%) was maximum at 0.1% hydroxybutyrate, while valerate at 0.1% and 0.3% was suitable for total polymer accumulation and 3HV monomer incorporation, respectively. Cosupplementation of hydroxybutyrate and valerate in the ratio of 3 : 1 led to the accumulation of PHA accounting for 54% of cell dry weight, which contained more than 50 mol% of 3HV monomer. Moreover, the biphasic cultivation conditions with hydroxybutyrate as cosubstrate have improved the quality as well as quantity of the accumulated copolymer significantly.