Phototrophic Growth and Accumulation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Purple Nonsulfur Bacterium Rhodopseudomonas palustris SP5212

Table 1

Effect of hydroxybutyrate and valerate on growth and PHA accumulation by R. palustris SP5212.

Concentration, % (w/v)	Growth, g/L	PHA, % CDW	Composition of PHA, Mol%
			3HB	3HV
Hydroxybutyrate
0.05	0.47	23.25	73.70	26.30
0.10	0.57	49.06	70.19	29.80
0.20	0.49	36.33	77.18	22.82
0.30	0.41	16.78	81.98	18.01
Valerate
0.05	0.52	15.19	87.44	12.56
0.1	0.50	29.84	85.64	14.36
0.2	0.44	20.31	71.13	28.87
0.3	0.39	17.43	62.39	37.61

The isolate was grown in acetate medium supplemented with hydroxybutyrate and valerate as cosubstrate under microaerophilic condition for 12 days at a light intensity of 10,000 lux, total PHA content was estimated according to the method of Ulmer et al. (1994) [18], and growth was determined by biomass dry weight.