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Journal of Renewable Energy
Volume 2013, Article ID 671545, 6 pages
Research Article

The Effects of Trace Elements on the Lipid Productivity and Fatty Acid Composition of Nannochloropis oculata

College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Zhejiang Province Key Laboratory of Biofuel, Hangzhou 310014, China

Received 7 December 2012; Revised 2 March 2013; Accepted 8 March 2013

Academic Editor: Yongsheng Chen

Copyright © 2013 Xiao Dou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effects of trace elements on the lipid productivity and fatty acid composition of Nannochloropis oculata (N. oculata) were studied. The results showed that trace elements had a strong influence on not only the lipid productivity but also the fatty acid composition. The addition of Fe3+, Zn2+, Mn2+, Mo6+, and EDTA and the deletion of Cu2+ and Co2+ can increase the lipid productivity. The optimum concentrations of the trace elements in the culture medium are 6 times of Fe3+ and EDTA, the same concentration of Zn2+, Mn2+, and Mo6+ as the control group, but the optimum medium has no Cu2+ or Co2+. Fe3+, Zn2+, Mn2+, Mo6+, and EDTA are indispensable during the EPA formation of N. oculata. The addition of Fe3+, Zn2+, Mn2+, Mo6+, and EDTA can strongly increase the content of EPA in the lipid of N. oculata, but the concentration of the trace elements had little influence on the level of EPA.