Synthetic Crossfeeding Cocultures in Yeast: Computational Model of Autoregulation and Design of a Tryptophan Export Device

Figure 4

Determination of tryptophan secretion for the engineered Trp-secretion I and Trp-secretion II strains. Fluorescence emission spectra (excitation at 295 nm) of −T culture media. Grey lines: −T medium with different added concentrations of tryptophan. Blue line: −T medium inoculated with H⁻ Trp-secretionI strain. Green line: −T medium inoculated with H⁻ Trp-secretionII strain. Red line: −T medium inoculated with a yeast carrying empty pCM182 plasmid.