Role for PKC 𝛿 in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells

Figure 10

The antioxidant Vitamin C blocks fenretinide-induced cleavage of PKC $\delta$ in RS4;11 cells. Western blot analysis was performed using antibody against PKC $\alpha$, PKC $\beta$I, PKC $\beta$II, PKC $\delta$, PKC $\epsilon$, p-JNK, JNK, BCL-${\text{X}}_L$, MCL-1, BCL2, and Tubulin on total lysate ($0.25\times {10}^6$ cell equivalents) from RS4;11 cells treated with vehicle (0.2% DMSO), 0.4 M Vitamin C (Vit C), 10 $\mu$M fenretinide (4-HPR), or 10 $\mu$M fenretinide after a 2 hour pretreatment with 0.4 M Vitamin C (Combo) for 24 hours.

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