Role for PKC 𝛿 in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells

Figure 12

Bryostatin-1 suppresses PKC $\delta$ expression in REH cells. Western blot analysis was performed using antibody against PKC $\delta$, PKC $\epsilon$, and Tubulin on total lysate ($0.25\times {10}^6$ cell equivalents) from REH cells and RS4;11 cells treated with vehicle (0.2% DMSO), 10 nM Bryostatin-1 (Bryo), fenretinide 4-HPR), or fenretinide after a 2 hour pretreatment with 10 nM Bryostatin-1 (Combo) for 24 hours. Due to differences in sensitivity to the drug, 10 $\mu$M fenretinide was used for REH cells and 1 $\mu$M fenretinide was used for RS4;11 cells.

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