Role for PKC 𝛿 in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells

Figure 14

Suppression of PKC $\delta$ promotes fenretinide-induced cell death in CCRF-CEM cells. Cell death of CCRF-CEM transfectant cells with control nonspecific (NS) shRNA and CCRF-CEM transfectant cells with PKC $\delta$ (PKC delta) shRNA treated with vehicle (0.1% DMSO) or fenretinide (4-HPR at 1 $\mu$M, 5 $\mu$M or 10 $\mu$M dose) for 24 hours was examined by trypan blue dye exclusion assay. Error bars represent the mean $\pm$ S.D. from three separate experiments. Statistically significant differences from cell viability in untreated cells (standard t-test; $P<.05$) are marked by “*”.