Role for PKC 𝛿 in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells

Figure 5

Caspase Inhibitors do not protect leukemia cell lines from fenretinide-induced apoptosis. Apoptosis of human leukemia derived RS4;11 and CCRF-CEM (CEM) cells treated with vehicle (0.2% DMSO) or fenretinide (10 $\mu$M 4-HPR) for 24 hours was examined using FACSCAN analysis of Annexin V stained cells. Where appropriate, cells were pretreated for 2 hours with 20 $\mu$M Caspase 8 inhibitor or 20 $\mu$M Caspase 9 inhibitor. Error bars represent the mean $\pm$ S.D. from three separate experiments. Statistically significant differences from cell viability in untreated cells (standard t-test; $P<.05$) are marked by “*”.