Suppression by Ghrelin of Porphyromonas gingivalis-Induced Constitutive Nitric Oxide Synthase S-Nitrosylation and Apoptosis in Salivary Gland Acinar Cells
Figure 7
Effect of iNOS inhibitor, 1400 W (14 W) on P. gingivalis LPS-induced cNOS S-nitrosylation. The acinar cells were treated with the LPS (100 ng/mL) or 14 W (30 M) + LPS and incubated for 16 hours in the presence of 100 ng/mL LPS. A portion of the cell lysates was processed by biotin switch procedure for protein S-nitrosylation and, along with the reminder of the lysates, subjected to SDS-PAGE, transferred to nitrocellulose and probed with anti-cNOS antibody. The immunoblots shown are representative of three experiments.
