Jak2-Independent Activation of Stat3 by Intracellular Angiotensin II in Human Mesangial Cells
Figure 7
Role of Jak2 in intracellular Ang II-induced activation of Stat3 in human mesangial cells. (a) A sample picture of Western blot analysis showing specific protein band for Jak2 (130 kDa), Stat3 (92 kDa), and b-actin (42 kDa) (loading control). (b) Densitometry analysis of Western blots revealed a significant increase in Stat3 protein expression in mesangial cells incubated with 1μM exogenous Ang II (NG + ex-Ang II) or transfected with Ang II (NG + t-Ang II) for 24 h. Treatment with Jak inhibitor I did not block increase in Stat3 protein in either NG + ex-Ang II or NG + t-Ang II group. Data are presented as mean ± SEM (). versus NG.
