Tyrosine Kinase Signaling in Kidney Glomerular Podocytes

Table 1

Summary of the studies on Nephrin and Neph1 phosphorylation under disease conditions.

	Antibodies	Phosphorylation in animal models	Phosphorylation in humann diseases	Reference
Nephrin	1208 and 1232	Decrease in PAN	Decrease in MCNS	[35]
	1208 and 1232		Slight decrease in MN	[36]
	1232	Decrease in PAN		[35]
	pTyr	Decrease in PAN		[37]
	1191/1208	Decrease in LPS		[38]
	1191/1208	Decrease in c-mip∗		[38]
	1191/1208	Increase in PAN		[30]
	1208	Increase in PS		[30]
	1208	Increase in PS		[33]
	pTyr	Increase in 27A∗∗		[25]
	pTyr	Increase in PHN		[39]
Neph1	pTyr	Increase in PAN		[40]
	637/638	Increase in PAN, PS		[41]

The numbers in the column “antibodies” mean the site of phosphorylation based on the mouse Nephrin sequence (accession number AAK38483). “1208 and 1232” means separate antibodies and “1191/1208” antibody recognizes both phosphorylaion sites. pTyr means commercial antiphosphotyrosine antibodies. PAN: puromycin aminonucleoside; LPS: lipoporysaccharide; PS: protamine sulfate; PHN: passive Heymann nephritis. MCN: minimal change nephrosis; MN: membranous nephropathy. ∗Mice overexpressing c-mip; ∗∗mice injected with 27A antibody against podocyte-specific 9-O-acetylated GD3 ganglioside.