Effect of PAR-2 Deficiency in Mice on KC Expression after Intratracheal LPS Administration

Figure 2

LPS stimulation of chemokines and TNF-α in alveolar and resident peritoneal macrophages. Macrophages from wild-type (PAR2^+/+, white bars) and PAR-2^−/− (black bars) mice were left untreated or stimulated with 100 ng/mL of LPS for indicated time periods. ELISAs on cell supernatants from alveolar macrophages were performed for KC (a), MIP-2 (b), and TNF-α (c). ELISAs on cell supernatants from resident peritoneal macrophages were performed for KC (d), MIP-2 (e), and TNF-α (f). 𝑛=3. *𝑃<0.05; **𝑃<0.001 by two way ANOVA posttest.

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