Table of Contents
Journal of Signal Transduction
Volume 2011 (2011), Article ID 507291, 9 pages
http://dx.doi.org/10.1155/2011/507291
Review Article

Regulation and Function of Syk Tyrosine Kinase in Mast Cell Signaling and Beyond

1Receptors and Signal Transduction Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
2Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14049-900, Brazil

Received 29 December 2010; Accepted 23 February 2011

Academic Editor: Peter van der Geer

Copyright © 2011 Rodrigo Orlandini de Castro. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The protein tyrosine kinase Syk plays a critical role in FcεRI signaling in mast cells. Binding of Syk to phosphorylated immunoreceptor tyrosine-based activation motifs (p-ITAM) of the receptor subunits results in conformational changes and tyrosine phosphorylation at multiple sites that leads to activation of Syk. The phosphorylated tyrosines throughout the molecule play an important role in the regulation of Syk-mediated signaling. Reconstitution of receptor-mediated signaling in Syk-/- cells by wild-type Syk or mutants which have substitution of these tyrosines with phenylalanine together with in vitro assays has been useful strategies to understand the regulation and function of Syk.